تعدد الأشكال للقاعدة المفردة rs2229569 مع التعبير الجيني للسلكتين L في الاناث العراقيات اثناء برنامج الاخصاب خارج الجسم الحي

المؤلفون

  • Raghad Hameed Rashid قسم علوم الحياة،كلية العلوم للبنات، جامعة بغداد ،بغداد ، العراق.
  • Asmaa M. Salih Almohaidi قسم علوم الحياة،كلية العلوم للبنات، جامعة بغداد ،بغداد ، العراق. https://orcid.org/0000-0002-6084-0349
  • Alia Hussain Ali قسم علوم الحياة،كلية العلوم للبنات، جامعة بغداد ،بغداد ، العراق.

DOI:

https://doi.org/10.21123/bsj.2024.8832

الكلمات المفتاحية:

التعبير الجيني ,تعدد الأشكال للقاعدة الواحدة rs2229569, العقم ,الاخصاب في المختبر,سلكتين L

الملخص

يُعرف العقم بأنه أحد مشاكل الصحة الإنجابية الرائدة في مناطق مختلفة من العالم. يعتبر الإخصاب في المختبرأحد أكثر علاجات العقم فعالية. في الدراسة الحالية ، تم تحديد دور جين SELL ، وخاصة تعدد الأشكال rs2229569 ، من حيث الانغراس ومستوى التعبير. اشتملت الدراسة على 67 انثى خضعن لدورة إخصاب في المختبر ، مقسمة إلى مجموعتين رئيسيتين: مجموعة الإناث التي فشلت عملية الزرع ومجموعة الإناث الناجحة في عملية الزرع. تم جمع عينات الدم من مجموعتي الاناث. بعد استخلاص الدنا من الدم وتضخيم  الحمض النووي عن طريق تفاعل البلمرة المتسلسل  ، تم إرسال العينات لتحليل التسلسل ، وتم تحديد SNP rs2229569  التي اظهرت  ثلاثة أنماط وراثية. كانت مجموعة الفشل منحرفه عن H.W.E. ، وكانت مجموعة النجاح في اتفاق مع H.W.E. وفقًا لقيم مربع كاي ((X2 = 11.60 1.58، تشير الفروق المعنوية في مجموعة الفشل إلى تأثير العقم في مجتمع الدراسة. تُظهر نسبة الأرجحية لـ rs2229569 أن أليل T كان (2.09) ، لذلك قد يمثل T allele أليل المخاطرة لعمليةفشل الزرع .أخيرًا ، أظهر جين SELL مستوى تعبيرًا منخفضًا لدى الاناث المصابات بفشل الزرع مقارنة بالاناث في مجموعات الزرع الناجحة.

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كيفية الاقتباس

1.
تعدد الأشكال للقاعدة المفردة rs2229569 مع التعبير الجيني للسلكتين L في الاناث العراقيات اثناء برنامج الاخصاب خارج الجسم الحي . Baghdad Sci.J [انترنت]. [وثق 18 مايو، 2024];21(9). موجود في: https://www.bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/8832